Imaging Cellular Components and Dynamics with Sub-Diffraction Resolution


Due to the near transparency of cells, optical imaging is well suited for studying live or intact cells.  Tagging cellular components with fluorophores dramatically extends these capabilities by providing molecular specificity and improved contrast. However, the light microscope is limited by diffraction to ~ 300 nm, whereas many important events for cell function and signaling involve interactions of proteins that occur at the ~ 10 nm scale. I will discuss methods for circumventing the diffraction limit using single molecule fluorescence imaging for both super-resolution and for imaging and quantifying protein-protein interactions in living cells. A sub-theme of the talk will be on the interesting challenges and opportunities available for the physicist working in this area.   The talk will not assume any prior knowledge of biological systems. 

8 Nov 2017
104 Gore Hall
Keith Lidke, University of New Mexico